Geri Dön

To reveal the genetic basis of dwarfism, with constructing an SNP-based genetic map basedon the progeny of the faba bean (Vicia faba L.) inbred lines, NV153_1DW_S3A2 andNV644_1_KASZTEL

Başlık çevirisi mevcut değil.

PDF İndir

  1. Tez No: 778605
  2. Yazar: SELİN EĞİLMEZ
  3. Danışmanlar: Belirtilmemiş.
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyomühendislik, Genetik, Ziraat, Bioengineering, Genetics, Agriculture
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2020
  8. Dil: İngilizce
  9. Üniversite: The University of Reading
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 114

Özet

Faba bean is a plant that plays a crucial role in human and animal nutrition, as well as the soil nitrogen content. Therefore, most of the faba bean breeding studies focus on increasing the yield. The dwarf gene has become common to increase the harvest index and the yield. In this research, it was aimed to find the genetic sequence of the dwarf gene in the faba bean. To determine that, as a genetic source NV153_1DW_S3A2 which has dwarf faba bean genome, normal flower colour and, dark hilum colour and NV644_1_KASZTEL (tall, white flower, pale hilum colour) inbred lines were crossed. To evaluate phenotypic traits, which are, flower colour, hilum colour, plant height in the F2 generation were recorded for phenotypic evaluation. The segregation ratio of F2 populations was tested with Chi-square tests for 3:1 single recessive gene inheritance, and the frequency distribution of the traits was founded. Moreover, in order to observe growth differences in dwarf and tall plants, the internode distance was recorded in the F2 generation during the cultivation process. To create a genetic map based on phenotypic traits young flowers' leaves were taken and analysed with SNP based PCR method with KASP assay. Then from 179 SNPs markers 112 individuals the genetic map was created by MapDisto 2.1. Two more maps were created both for the quantitative trait and qualitative traits. From the phenotypic traits map, the synteny was explored, the interval of the gene that was responsible for dwarfism, flower colour, and hilum colour was founded according to collinearity between the faba bean chromosome with Medicago. It was concluded with the Medtr3g092830 gene responsible for the colour of the flowers. While Vf_Mt2g012470_001 and Vf_Mt2g013690_001 was assumed as a candidate gene for hilum colour, for dwarfism the previous gene of Vf_Mt2g104110_001 was evaluated to as candidates. 13 However, according to the QTL map, it was founded that the linkage group two had a“tall”QTL peak in the vicinity of the markers that were already identified as flanking the dwarfing gene.

Özet (Çeviri)

Özet çevirisi mevcut değil.

Benzer Tezler

  1. Tez No

    692149

    Çukurova bölgesindeki keratokonus olgularında VSX1 ve LOX genlerinin dizileme yöntemi ile analizi

    Sequencing analysis of VSX1 and LOX genes in cases with keratoconus in the Cukurova region

    ASTAN IBAYEV

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2021

    Göz HastalıklarıÇukurova Üniversitesi

    Göz Hastalıkları Ana Bilim Dalı

    PROF. DR. MELTEM YAĞMUR

  2. Tez No

    348724

    Bioinformatics approaches to associate single nucleotide polymorphisms with human diseases according to their pathway related context

    Tek nükleotid polimorfizmlerini yolaklar üzerinden insan hastalıkları ile ilişkilendirmek için biyoinformatik yöntemler

    BURCU GÜNGÖR

    Doktora

    İngilizce

    İngilizce

    2012

    Bilgisayar Mühendisliği Bilimleri-Bilgisayar ve KontrolSabancı Üniversitesi

    Biyoloji Bilimleri ve Biyomühendislik Ana Bilim Dalı

    DOÇ. DR. OSMAN UĞUR SEZERMAN

  3. Tez No

    477233

    Finansal yatırım kararlarında genetik etkiler: Duygusal ön yargılar analizi

    Genetic effects on financial investment decisions: Emotional biases analysis

    SEZEN GÜNGÖR

    Doktora

    Türkçe

    Türkçe

    2017

    İşletmeTrakya Üniversitesi

    İşletme Ana Bilim Dalı

    DOÇ. DR. ENGİN DEMİREL

  4. Tez No

    560923

    Yeni nesil dizileme sisteminin tanıda uygulanması ve sonuçların biyoinformatik yöntemlerle değerlendirilmesi

    Application of new generation sequencing system to diagnosis and approach of bioinformatic tools

    NARMIN AHMADOVA

    Yüksek Lisans

    Türkçe

    Türkçe

    2019

    Bilim ve TeknolojiEge Üniversitesi

    Biyomedikal Teknolojiler Ana Bilim Dalı

    PROF. DR. AFİG BERDELİ

  5. Tez No

    644808

    Pulmoner arteriyel hipertansiyonlu hastalarda smad1 (smad family member 1) geninin polimorfizmi ve ekspresyon düzeyinin araştırılması

    Investigation of the polymorphism and expression level of the smad1 (smad family 1 member) gene in patients with pulmonary arterial hypertension

    ADNAN SELİM KİMYON

    Yüksek Lisans

    Türkçe

    Türkçe

    2020

    Tıbbi BiyolojiMersin Üniversitesi

    Tıbbi Biyoloji Ana Bilim Dalı

    PROF. DR. NURCAN ARAS

Geri Dön