Novel chemosensitizer effect of the antibacterial agent dmc-deb005 through inhibition of autophagy
Başlık çevirisi mevcut değil.
- Tez No: 838535
- Danışmanlar: DR. KATALİN KOVáCS
- Tez Türü: Yüksek Lisans
- Konular: Mikrobiyoloji, Moleküler Tıp, Tıbbi Biyoloji, Microbiology, Molecular Medicine, Medical Biology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2024
- Dil: İngilizce
- Üniversite: Debreceni Egyetem
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 32
Özet
Autophagy is the process of self-eating which involves the degradation of cellular elements and recycling of the building blocks. A basal level of autophagy is necessary to keep cells free of damaged proteins and organelles. Defective autophagy is observed in many human diseases. However, autophagy plays a dual role in tumorigenesis and in cancer treatment responsiveness. While in the early stages of cancer development, autophagy has a predominantly protective role restricting carcinogenesis, in the more advanced phases, it stimulates cancer growth and spreading. It has been proposed that autophagy inhibitors may potentiate the anticancer effect of various chemotherapeutic agents. Previously we carried out a DALGreen-based high-content screening of 775 FDA-approved compounds and identified an antibacterial agent (DMC-Deb005) as a drug that reduces the rate of autophagy in A549 cells. To better understand the effect of autophagy on cancer, we applied this drug to the A459 adenocarcinoma cell line in a combined treatment with 5-fluorouracil and etoposide as anticancer agents. We measured cell viability using MTT assay. Combined treatment resulted in reduced viability of cancer cells, compared to treatments with anticancer agents only. The combined treatment also decreased the long-term proliferation rate of cells measured with sulphorodamin B assay. We also tested the effect of the compound in a 3D cell culture (spheroid) assay. We observed that the cells with combined treatment displayed improper formation of cellular spheroids and more pronounced spheroid disruption have also been observed. In conclusion, DMC-Deb005 and the anticancer agents have synergistic effects on cancer cell death in our model. Supervisors: Dr. Kovács Katalin, Prof. Dr. Virág László
Özet (Çeviri)
Özet çevirisi mevcut değil.
Benzer Tezler
- Nsaid'ların (non-steroidal anti-inflammatory drug) topikal uygulamaları için yeni jel formulasyonlarının hazırlanması: Kontrollü ilaç salım potansiyellerinin araştırılması
The prepation of new gel formulation for topical aplications of nsaids (non-steroidal anti-inflammatory drug): Investigation of potential controlled drug release
ŞEREF KAPLAN
- Novel cellulase enzyme production towards biofuels sector by recombinant bacterium Escherichia coli
Biyoyakıt sektörüne yönelik yeni nesil selülaz enzimlerinin rekombinant Escherichia coli bakterisinde üretimi
ZEHRA YILDIRIM
Yüksek Lisans
İngilizce
2015
BiyomühendislikHacettepe ÜniversitesiBiyomühendislik Ana Bilim Dalı
YRD. DOÇ. DR. EDA ÇELİK AKDUR
- p-dimetilanilinazometinfenollerin oksidatif kondenzasyon polimerizasyonu
Oxidative condensation polymerization of p-dimethylanilineazomethinephenols
NURAY YILMAZ BARAN
- Novel vision based estimation techniques for the analysis of cavitation bubbles
Kavitasyon kabarcıklarının analizi için görmeye dayanan özgün kestirim teknikleri
GÖKHAN ALCAN
Yüksek Lisans
İngilizce
2015
Mekatronik MühendisliğiSabancı ÜniversitesiMekatronik Mühendisliği Ana Bilim Dalı
PROF. DR. MUSTAFA ÜNEL
- Novel near-ir photosensitizers for photodynamic therapy & designing heavy atom free photosensitizers for the photodynamic therapy
Fotodinamik terapi için kızıl ötesi ışığı soğurabilen yeni fotoduyarlaştırıcı ve ağır atom içermeyen fotoduyarlaştırıcıların tasarlanması
BİLAL KILIÇ
Doktora
İngilizce
2016
Kimyaİhsan Doğramacı Bilkent ÜniversitesiMalzeme Bilimi ve Nanoteknoloji Ana Bilim Dalı
PROF. DR. ENGİN UMUT AKKAYA
