Geri Dön

Gender differences in the gene expression profile of adipose tissue in an insulin resistant mouse model (irs2-/-)

Başlık çevirisi mevcut değil.

  1. Tez No: 912462
  2. Yazar: DEMET INCİ
  3. Danışmanlar: Belirtilmemiş.
  4. Tez Türü: Yüksek Lisans
  5. Konular: Belirtilmemiş.
  6. Anahtar Kelimeler: adipose tissue, diabetes, gender, gene expression, irs2
  7. Yıl: 2019
  8. Dil: İngilizce
  9. Üniversite: Universitat de València
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 60

Özet

Insulin is an essential anabolic hormone involved in cell growth, proliferation, and development. Defects in this hormone production and function lead to diabetes. The insulin receptor substrate-2 (Irs2) coordinates insulin action in pancreatic cell function and peripheral tissues, including as a main target adipose tissue metabolism. Irs2 knockout (Irs2- /-) mouse model has shown to develop type 2 diabetes, thus we decided to investigate adipose tissue of this mouse model at the gene expression level including insulin signaling pathway (Irs1, Irs2, InsR, Igf1, Igf1R) and other signaling pathways that could be affected by Irs2 gene ko including FGF signaling (Fgf7, Fgf21), inflammation and obesity (IL-6, Tgfβ, Opn, Pparγ, Lep, LepR), and the mitochondrial gene Ucp2. Since biological differences between males and females cause changes in body metabolism, especially in the adipose tissue, we decided to include this gender variant, as well as the putative differences between fed and fasting state. In order to conduct a proper gene expression analysis, we selected from four putative genes (Hprt, 18s, Gapdh, and Ubc) the one that better adjusted to a housekeeping reference gene, selecting for further analysis the candidate Hprt. Gene expression studies have revealed a differential profile for male and females in fed conditions in which males were overexpressing Irs2, Igf1R, Lep, Fgf21, and Opn when compared to females, and females were overpressing Irs1, Fgf7, Il6, and LepR when compared to males, in normal condition. When insulin resistance was induced (Irs2-/- ko model), males compensated the absence of Irs2 by overexpressing other ins signaling pathways like Irs1, InsR or Igf1, while females decreased Irs1, Igf1R and Fgf7, Ucp2 and LepR expression, while increased Lep signal. Further, significant differences were observed between fed and fasting state (Irs1, Il6, Lep, LepR). Overall, our results highlight the importance of gender in gene expression analysis, especially in a situation of insulin resistance in order to look for proper markers that could be involved in the diagnosis, clinic, and treatment of type 2 diabetes in the future.

Özet (Çeviri)

Özet çevirisi mevcut değil.

Benzer Tezler

  1. Genome wide quantitative trait loci analysesof body composition and obesity

    Vücut kompozisyonu ve obezitenin genom çapında kantitatif özellik lokusları analizleri

    AYÇA DOĞAN

    Doktora

    İngilizce

    İngilizce

    2011

    GenetikHumboldt-Universität zu Berlin

    Moleküler Biyoloji ve Genetik Ana Bilim Dalı

    PROF. DR. GUDRUN A. BROCKMANN

  2. Growth performance and tissue fatty acid change in rainbow trout (Oncorhynchus mykiss) feed with different energy levels

    Farklı enerji içeriğine sahip yemlerle beslenen gökkuşağı alabalıklarında (Oncorhynchus mykiss) büyüme performansı ve doku yağ asidi değişimi

    DOAA BAHJAT ALWAN KRAIEI

    Yüksek Lisans

    İngilizce

    İngilizce

    2024

    BiyoteknolojiÇukurova Üniversitesi

    Biyoteknoloji Ana Bilim Dalı

    PROF. DR. ORHAN TUFAN EROLDOĞAN

  3. Akciğer adenokarsinomu ve malign plevral mezotelyoma ayırıcı tanısında gen ekspresyon farkının araştırılması

    Evaluation of gene expressions in the diagnosis of lung adenocarsinoma and malignant pleural mesothelioma

    GÖKÇEN ÖMEROĞLU ŞİMŞEK

    Doktora

    Türkçe

    Türkçe

    2016

    Moleküler TıpDokuz Eylül Üniversitesi

    Temel Onkoloji Ana Bilim Dalı

    PROF. DR. HÜLYA ELLİDOKUZ

  4. Unraveling the apomixis-related genes in Boechera genus: BM, APOLLO, and LEC2

    Boechera cinsinde apomiksi ile ilişkili genlerin ortaya çıkarılması: BM, APOLLO, ve LEC2

    BUKET ÜNER

    Yüksek Lisans

    İngilizce

    İngilizce

    2023

    BiyolojiÇanakkale Onsekiz Mart Üniversitesi

    Moleküler Biyoloji ve Genetik Ana Bilim Dalı

    PROF. DR. KEMAL MELİH TAŞKIN

  5. Germinal merkez kaynaklı olan ve olmayan diffüz büyük b hücreli lenfomalarda c-myc translokasyonu sıklığı

    Frequency of c-myc translocation in diffuse large b-cell lymphomas with and without germinal center

    ŞENAY YILDIRIM

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2011

    PatolojiAkdeniz Üniversitesi

    Patoloji Ana Bilim Dalı

    PROF. DR. BAHAR AKKAYA