Alkalen proteaz üreticisi yerel Bacillus soylarının izolasyonu, nitelendirilmesi ve enzim üretiminin optimizasyonu
The isolation, characterization of the local bacillus strains and the optimization of the enzyme production
- Tez No: 150359
- Danışmanlar: PROF.DR. FÜSUN GÜMÜŞEL
- Tez Türü: Yüksek Lisans
- Konular: Biyoloji, Biology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2004
- Dil: Türkçe
- Üniversite: Gebze Yüksek Teknoloji Enstitüsü
- Enstitü: Mühendislik ve Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Biyoloji Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 86
Özet
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Özet (Çeviri)
A \ $».>*.*A sut v SUMMARY Vi^*,- Proteases are hydrolytic enzymes which catalyzes the hydrolysis of the proteins. Alkaline proteases, sub-group of serine proteases (E.C. 3.4.21), which are stable under alkaline pH and high temperature are widely used in detergent, leather, food, silk and paper industries. Although alkaline proteases are produced by a wide range of microorganisms such as bacteria, fungi and molds, Bacillus species are the main preferred sources for these enzymes. Nowadays, the number of researches targeting the isolation of novel alkaline protease producing Bacillus species has been accelerated. In this thesis work, performed with the collaboration of Enzyme Molecular Genetics Group; Department of Biology, Gebze Institute of Technology and Bacteriology Laboratory, Pendik Medical Centre, Kızılay, the isolation and the identification of Bacillus species according to the morphological and biochemical properties and selection of alkaline protease producing strains among these isolates had been aimed. Among the locally isolated Bacillus species, 28 isolates were selected according to the radius of the clear zones around the colonies due to the hydrolysis of the casein in the skim-milk containing selective medium and then, the closest species names of these 28 Bacillus isolates were proposed according to the morphological and biochemical properties by performing classical microbiological techniques. For the nine strains chosen as the best enzyme producers among 28 isolates in liquid, growth conditions for optimal enzyme production were determined and, also, enzyme productivity parallel to cell growth was analysed. Then, these nine isolates were re-identified by API CHB/E and VITEK Bacillus kits in order to confirm the species names proposed at the pre- identification step. Finally, the species names of these nine isolates were given by comparing the results obtained from the three methods. For the isolates, which were not identified by these three methods, 16S rDNA sequence analysis studies have been started. In addition, the pre-characterization of the alkaline protease produced by strain LI 3 was done; and the optimal temperature, the optimal pH and pH stability range were found to be 50-55 °C, 1 1.5 and 5.5-12, respectively.
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