Isolation, purification, characterization, mode of action, and amino acid sequence of bifidocin B and lactococcin R
Başlık çevirisi mevcut değil.
- Tez No: 400650
- Danışmanlar: PROF. DR. MICHEAL G. JOHNSON
- Tez Türü: Doktora
- Konular: Mikrobiyoloji, Microbiology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1998
- Dil: İngilizce
- Üniversite: Unıversıty Of Arkansas
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
Özet yok.
Özet (Çeviri)
Ten dairy products and ten fresh vegetables products were researched for bacteria that produce bacteriocins. Also, Bifidobacteria species from ATCC (11863, 29521), and NCFB (1453, 1454, 1455) were examined for possible production of bacteriocins. Only Bifidobacterium bifidum NCFB 1454 and a radish isolate, Lactococcus lactis subsp. cremoris R, produced bacteriocins designated bifidocin B (BB) and lactococcin R (LR), respectively. BB and LR were sensitive to some proteolytic enzymes tested. Both bacteriocins were resistant to organic solvents, pH, or heating (90-121°C) and were active against Listeria, Enterococcus, Bacillus, Micrococcus, LacwbaciUus, Leuconostoc and Pediococcus species tested. Also, LR was inhibitory to Clostridium, Staphylococcus and Streptococcus species. Their activity (1,280 AU/ml) on L. monocytogenes was bactericidal, reducing colony counts by 99.9% in 3-h. BB and LR were purified to homogeneity by a simple purification procedure including freeze-drying, Micro-Cel adsorption/desorpcion and cation«exchangeT chromatography. These bacteriocins caused sensitive cells to lose intracellular K+ ions, UV-absorbing materials and become more permeable to ONPG. Bacteriocins adsorbed to the gram-positive bacteria but not gram-negative bacteria tested. Several salts inhibited their binding. Also, addition of purified lipoteichoic acid to sensitive cells and methanol:chloroform plus hot 20% TCA treatment on cell wall preparations blocked bacteriocin adsorption. The N-terminal amino acid sequence of BB yielded
Benzer Tezler
- Synthesis and characterization of pentacarbonyl (vinylferrocene) metal (O) complexes (metal=chromium, molybdenum, tungsten)
Pentakarbonil (vinilferrosen) metal (O)'ın (metal=krom, molibden, volfram) sentezi ve tanımlanması
NAZLAN DEMİR
- Isolation, purification and characterization of catalase from Phanerochaete Chrysosporium
Katalaz enziminin Phanerochaete Chrysosporium dan izolasyonu, saflaştırılması ve karakterizasyonu
BERNA KAVAKÇIOĞLU
- Isolation, purification and characterization of secondary metabolites from nigella arvensis var. involucrata
Nigella arvensis var. involucrata'dan sekonder metabolitlerin izolasyonu, saflandırılması ve karakterizasyonu
KENAN BIÇAK
- Isolation, purification, characterization of nitrogen-fixing bacteria from potato rhizosphere soils in Türkiye
Türkiye'deki patates rizosfer topraklarından azot bağlayıcı bakterilerin izole edilmesi, saflaştırılması ve karakterizasyonu
SENA USTA
Yüksek Lisans
İngilizce
2025
Gebze Teknik ÜniversitesiMoleküler Biyoloji ve Genetik Ana Bilim Dalı
Yükleniyor...
- Isolation, partial purification and characterization of alpha amylase produced by streptomyces sp
Streptomyces sp tarafından üretilen alfa amilazın izolasyonu, kısmi saflaştırılması ve karakterizasyonu
AYŞEGÜL ÇALIŞKAN