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Characterization of a new type three secretion effector in Xanthomonas cynarae

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  1. Tez No: 403105
  2. Yazar: SERHAT KARA
  3. Danışmanlar: PROF. DR. JEFFREY B. JONES
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyoloji, Botanik, Tıbbi Biyoloji, Biology, Botany, Medical Biology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2016
  8. Dil: İngilizce
  9. Üniversite: University of Florida
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 77

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Özet (Çeviri)

X. gardneri and X. cynarae are identical to each other based on multilocus sequence analyses (MLSA) using house-keeping genes (dnaK, fyuA, gyrB, and rpoD). Based on other work, both species have greater than 99% average nucleotide identity and should be considered as the same species. Given that X. gardneri and X. cynarae are closely related, cross-infection is likely to be occurring between these two species. In this study we demonstrated that infiltration of bacterial suspensions adjusted to low concentrations of the two bacterial species into pepper leaves revealed that the bacteria reached similar bacterial populations and produce similar symptoms. In artichoke infiltration of bacterial suspensions adjusted to low concentrations of X. cynarae and one X. gardneri strain showed that the bacteria reached similar bacterial populations. However, following spray inoculation of artichoke leaves, X. cynarae produced typical lesions, whereas the X. gardneri strain produced no obvious symptoms. In tomato, X. cynarae elicited a hypersensitive response (HR) as quantified by measuring electrolyte leakage and internal bacterial populations. We undertook to study the factor(s) in Xanthomonas cynarae that elicits an HR in tomato. Molecular techniques including creating a genomic library and subcloning were used to identify a unique effector in X. cynarae, designated xopAY, that is delivered via the type three secretion system and that was shown when expressed in X gardneri and infiltrated at high bacterial concentrations into tomato leaflets to be associated with rapid cell death. However, final internal bacterial populations were unaffected indicating that it is not a hypersensitive reaction. When this gene was mutated in X. cynarae, electrolyte leakage was significantly reduced indicating that this gene does contribute in X. cynarae to rapid cell death. We are in the process of identifying the other factor(s) associated with incompatibility of X. cynarae on tomato.

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