The metabolism of plant glucosinolates by gut bacteria
Başlık çevirisi mevcut değil.
- Tez No: 403342
- Danışmanlar: Prof. ARJAN NARBAD, Dr. MELINDA MAYER, Prof. RICHARD MITHEN
- Tez Türü: Doktora
- Konular: Biyoteknoloji, Genetik, Gıda Mühendisliği, Biotechnology, Genetics, Food Engineering
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2017
- Dil: İngilizce
- Üniversite: University of East Anglia
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 240
Özet
Özet yok.
Özet (Çeviri)
Glucosinolates found in cruciferous vegetables are degraded by plant myrosinases into bioactive isothiocyanates (ITCs) which have been recognised as potent anticancer compounds. During cooking, plant myrosinases are heat inactivated so ITC production is dependent on the myrosinase-like enzymes produced by the gut bacteria. This study is focused on investigating glucosinolate metabolism by the human gut bacteria and identifying the enzymes that play a crucial role. Human gut bacteria that were previously reported to metabolise glucosinolates were investigated in this study. In addition, 98 more human gut strains were isolated using a glucoraphanin enrichment method. It was hypothesised that bacterial myrosinases are β-glucosidases with specificity for glucosinolates. To identify the first bacterial myrosinase from the human gut, four putative β-glucosidases from Enterococcus casseliflavus CP1 and Escherichia coli FI10944 were cloned and heterologously expressed in E. coli. An alternative approach using a combination of ion exchange chromatography and gel filtration was also carried out to identify the bacterial myrosinase of E. coli FI109444. It has been reported that some gut bacteria require a reduction step to metabolise methylsulfinylalkyl glucosinolates (such as glucoraphanin) that converts them into methylthioalkyl glucosinolates (such as glucoerucin) to produce ITCs. To identify the responsible reductase, candidate reductase genes were cloned and expressed in E. coli. Methionine sulfoxide reductase B (MsrB) from Escherichia coli VL8 and Lactobacillus agilis R16 was found to reduce glucoraphanin to glucoerucin under the conditions tested. A bacterial myrosinase of Citrobacter WYE1 of soil origin was previously identified and myrosinase activity of this enzyme was characterised using cell-free extracts. In this study the myrosinase gene was heterologously expressed in E. coli to allow purification and characterisation. The recombinant enzyme showed activity against several glucosinolate substrates and protein was produced for crystallographic studies.
Benzer Tezler
- Metal etkisi uygulanarak yetiştirilen roka (Eruca sativa) bitkisinin antioksidan aktivitesinin belirlenmesi
Determination of antioxidant activity of arugula (Eruca sativa) plant grown with metal effect
ESRA KOCABAY
- Sülforafanın 769-P insan böbrek kanser hücre dizisinde apoptoz üzerine etkisi ve hücre migrasyonunun inhibisyonu
Effect of sulforaphane on apoptosis in 769-P human kidney cancer cell line and inhibition of cell migration
GÜZİN GÖKAY
Yüksek Lisans
Türkçe
2019
BiyolojiBezm-i Alem Vakıf ÜniversitesiBiyoteknoloji Ana Bilim Dalı
DOÇ. DR. AYDAN DAĞ
- Transcriptional profiling in Arabidopsis thaliana SLIM1 mutant exposed to boron toxicity
Bor toksisitesine maruz kalan Arabidopsis thaliana SLIM1 mutantında transkripsiyonel profilleme
BEDRİYE NİHAN YURTSEVEN
Yüksek Lisans
İngilizce
2024
BiyoteknolojiBaşkent ÜniversitesiMoleküler Biyokimya ve Genetik Ana Bilim Dalı
DOÇ. DR. CEYHUN KAYIHAN
- Tıbbi önemi olan bitkilerin sekonder metabolitlerinde miRNA'ların düzenleyici rollerinin belirlenmesi
Determination of regulatory roles of miRNAs in secondary metabolites of medicinally important plants
PERİHAN YİĞİT
Yüksek Lisans
Türkçe
2024
BiyolojiYıldız Teknik ÜniversitesiMoleküler Biyoloji ve Genetik Ana Bilim Dalı
DOÇ. DR. SEVGİ MARAKLI
- Tetralon ve kinazolin içeren yeni konjuge sistemli maddelerin dizaynı, sentezi ve yenilikçi uygulamaları
Design, synthesis, and innovative applications of new conjugated system compounds containing tetralone and quinazoline
ÖZCAN GÜLEÇ