Biber genotiplerinde farklı karbonhidrat kaynaklarının ve gümüş nitratın androgenez performansına etkilerinin araştırılması
Investigation of differend carbohydrate source and silver nitrate to effect of androgenez performance in pepper genotype
- Tez No: 420595
- Danışmanlar: PROF. DR. HÜLYA İLBİ
- Tez Türü: Yüksek Lisans
- Konular: Ziraat, Agriculture
- Anahtar Kelimeler: Pepper, Anther culture, Maltose, Sucrose, Genotype, Nutrient media, AgNO3, Pepper, Anther culture, Maltose, Sucrose, Genotype, Nutrient media, AgNO3
- Yıl: 2015
- Dil: Türkçe
- Üniversite: Ege Üniversitesi
- Enstitü: Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Bahçe Bitkileri Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 79
Özet
The aim of this research is to determine the most appropriate nutrient media content in anther culture. Moreover, the effect of using maltose as carbohydrate source and silver nitrate in nutrient media was studied in this thesis. The anthers of four different pepper varieties (Asena, Athena, Naomi and Rain Nil) were cultured in six different nutrient culture media combination with different sugar and various concentration of AgNO3.Callus and embryo in all the genotypes were obtained but plant regeneration was observed only in Asena (capia type) and Rain Nil (bell type) genotypes. M10 and M15 nutrient media which include maltose as a carbohydrate source gave the highest result for callus, embryo, and plant regeneration. However the M0 nutrient media without silver nitrate was not succesfull for adrogenesis compare to the other media that include maltose. The sucrose as a carbohydrate source in nutrient media also gave succesfull results depending on the genoytpes and AgNO3 composition. As a result, Asena genotype gave the best adrogenesis performance in maltose media with silver nitrate (10mg/l 15mg/l). Results are evaluated in terms of variety-nutration media the Asena genotype in M10 (71,1%) and M15 (74,2%) cultured media gave the best interaction for callus regeneration. In terms of the culture media for embryo regeneration, M15 (66,2 %) cultured media gave the most succesfull results and in genotypes the best success was achieved from Asena (60,1) genotype. Plant regeneration were oblained from the Rain Nil and the Asena genotypes. M15 cultured media (12,7%) and the Rain Nil genotype (8,4%) showed success for oblained plants from the pepper anthers. However, the best interactions for plant regeneration was derived from M15 cultured media for Rain Nil genotype (28,5%).
Özet (Çeviri)
The aim of this research is to determine the most appropriate nutrient media content in anther culture. Moreover, the effect of using maltose as carbohydrate source and silver nitrate in nutrient media was studied in this thesis. The anthers of four different pepper varieties (Asena, Athena, Naomi and Rain Nil) were cultured in six different nutrient culture media combination with different sugar and various concentration of AgNO3. Callus and embryo in all the genotypes were obtained but plant regeneration was observed only in Asena (capia type) and Rain Nil (bell type) genotypes. M10 and M15 nutrient media which include maltose as a carbohydrate source gave the highest result for callus, embryo, and plant regeneration. However the M0 nutrient media without silver nitrate was not succesfull for adrogenesis compare to the other media that include maltose. The sucrose as a carbohydrate source in nutrient media also gave succesfull results depending on the genoytpes and AgNO3 composition. As a result, Asena genotype gave the best adrogenesis performance in maltose media with silver nitrate (10mg/l 15mg/l). Results are evaluated in terms of variety-nutration media the Asena genotype in M10 (71,1%) and M15 (74,2%) cultured media gave the best interaction for callus regeneration. In terms of the culture media for embryo regeneration, M15 (66,2 %) cultured media gave the most succesfull results and in genotypes the best success was achieved from Asena (60,1) genotype. Plant regeneration were oblained from the Rain Nil and the Asena genotypes. M15 cultured media (12,7%) and the Rain Nil genotype (8,4%) showed success for oblained plants from the pepper anthers. However, the best interactions for plant regeneration was derived from M15 cultured media for Rain Nil genotype (28,5%).
Benzer Tezler
- Bazı biber genotiplerinde anter kültürü ile haploid embriyo uyartımında embriyo kalitesinin artırılmasına yönelik bazı uygulamalar
Different applications to improve the embryo quality on stimulation of embryo by anther culture in some pepper genotypes
HATIRA TAŞKIN
Yüksek Lisans
Türkçe
2005
ZiraatÇukurova ÜniversitesiBahçe Bitkileri Ana Bilim Dalı
PROF.DR. SAADET BÜYÜKALACA
- Endonezya kökenli bazı biber genotiplerinde anter kültürü yoluyla dihaploidizasyon
Dihaploidization in some Indonesian pepper genotypes via anther culture
ZULFA AZ ZAHROH ZULFA AZ ZAHROH
Yüksek Lisans
İngilizce
2022
BiyoteknolojiSelçuk ÜniversitesiBahçe Bitkileri Ana Bilim Dalı
PROF. DR. ERTAN SAİT KURTAR
- Kuraklık stresinin bazı biber genotiplerinde meydana getirdiği etkilerin morfolojik, fizyolojik, biyokimyasal ve moleküler özellikler bakımından incelenmesi
Investigation of the effects of drought stress on some pepper genotypes in terms of morphologic, physiological, biochemical and molecular properties
OĞUZHAN ARAZ
- Capsicum chinense türüne ait biber genotiplerinde tohum canlılığı üzerine sıcaklığın etkisinin incelenmesi
Investigation of the effect of temperature on seed viability inCapsicum chinense genotypes
BİRCAN GÖKPINAR
Yüksek Lisans
Türkçe
2021
ZiraatOndokuz Mayıs ÜniversitesiBahçe Bitkileri Ana Bilim Dalı
PROF. DR. AHMET BALKAYA
- Farklı biber (Capsicum annuum L.) genotipleri üzerinde shed-mikrospor kültürü tekniğinin uygulanması ve mikrospor gelişim aşamalarının belirlenmesi
Application of shed-microspore culture technique on different pepper (Capsicum annuum L.) genotypes and identification of microspore development stages
FARUK BERKAY ERİM
Yüksek Lisans
Türkçe
2019
BiyoteknolojiAkdeniz ÜniversitesiBahçe Bitkileri Ana Bilim Dalı
PROF. DR. AHMET NACİ ONUS