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Farklı düzeylerde ayçiçek yağı içeren yemlerle beslenen kazların serum ve yağ dokularında esansiyel yağ asitlerinin HPLC ile analizi ve canlı ağırlık üzerindeki etkisinin araştırılması

High performance liquid chromatographic analysis of serum and adipose tissues esantial fatty acids in gease fed diets containing different levels of sunflower oil and the investigation of the effects of diets on live wei

  1. Tez No: 48417
  2. Yazar: NECATİ UTLU
  3. Danışmanlar: DOÇ. DR. NCATİ KAYA
  4. Tez Türü: Doktora
  5. Konular: Biyokimya, Veteriner Hekimliği, Biochemistry, Veterinary Medicine
  6. Anahtar Kelimeler: Bitkisel yağlar, Hayvan yemleri, Kazlar, Kromatografi-yüksek basınçlı-sıvı, Yağ asitleri-esansiyel, Plant oils, Animal feeds, Geese, Chromatography-high pressure-liquid, Fatty acids-essential
  7. Yıl: 1996
  8. Dil: Türkçe
  9. Üniversite: Kafkas Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Biyokimya Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: Belirtilmemiş.

Özet

57 deneme gruplarında sırasıyla 8.86 ± 0.79, 7.36 ±1.17, 8.39 ± 0.99 ve 8.64 ±1.18 nmol/g. YA bulundu. Seruîn linoleik, linolenik ve araşidonik asitler arasındaki korelasyon katsayıları istatistiki olarak önemsiz bulundu. Derialtı yağ dokusu ise II. grupta linoleik- lİnolenik,, linoleik-araşidonik arasında negatif ve V. grupta ise linoleik-araşidonik arasında pozitif korelasyon bulundu. Abdominal yağ dokusunda da, IV. grupta linoleik-araşidonik arasında pozitif korelasyon derecesinde önemliydi (p<0.05). Ön çalışmayla, kazlar için, literatürlerde karşılaşılmayan serum, derialtı ve abdominal dokudaki esansiyel yağ asitlerinin normal değerleri tesbit edildi.59 significant statistically between groups I-IV, III-V and IV-V.(p<0.0 1). Average values of serum linoleic acid in the control group 35.09 ± 1.79 nmol/ml, in experimental groups 40.77 ± 3.32, 47.32 ± 4.09, 63.50 ± 7.05 and 27.48 ± 2.35 nmol/ml respectively, and the difference between groups I-IV, II-IV, II- V, ni-IV, III- V and IV-V. was significant (p<0.01). Linolenic acid in the control group 22.90 ± 2.27 nmol/ml, in experimental groups 24.20 ± 2.09, 36.52 ± 3.76, 49.18 ± 4.26 and 20.66 ± 2.56 nmol/ml respectively, and the difference between groups I-in, I-IV, II- in, H-IV, in-IV, III-V and IV-V. was significant (p<0.01). Arachidonic acid in the control group 10.57 ± 0.86 nmol/ml, in experimental groups 10.70 ± 0.83, 9.04 ± 0.64, 5.80 ± 0.90 and 9.48 ±1.11 nmol/ml respectively, and the difference between groups I-m, I-IV, H-in, II-IV, in-IV, m-V and IV-V. was found to be significant (pO.01). Average values of linoleic acid in subcutan fatty tissue in the control group 14.74 ± 1.8 nmol/g wet wt, in experimental groups 16.23 ± 1.16, 23.42 ± 2.85, 34.63 ± 3.57 and 8.95 ± 0.82 nmol/g wet wt respectively, and the difference between groups I-in, I-IV, II-III, H-IV, H-V, in-IV and in-V. was found to be significant (p<0.01). Linolenic acid in the control group 8.42 ± 0.81 nmol/g wet wt, in experimental groups 8.90 ± 0.70, 17.21 ± 1.33, 22.92 ± 2.31 and 6.98 ± 0.37 nmol/g wet wt respectively, and the difference between groups I-III, I-IV, II-III, II-IV, III- IV, III-V and IV-V. was significant (p<0.01). Arachidonic acid in the control group 5.59 ± 0.60 nmol/g wet wt in experimental groups 5.13 ± 0.67, 5.45 ± 0.57, 4.20 ± 0.42 ve 6.76 ± 0.84 nmol/g wet wt respectively. Average values of linoleic acid in abdominal fatty tissue in the control group 19.58 ± 2.37 nmol/g wet wt, in experimental groups 28.27 ± 4.75, 49.0 ± 11.27, 61.01 ± 9.98 and 18.40 ± 1.15 nmol/g wet wt respectively, and the difference between groups I-III, I-IV, II-IV and IV-V. was found to be significant (pO.01). Linolenic acid in the control group 8.95 ± 0.76 nmol/g wet wt, in experimental57 deneme gruplarında sırasıyla 8.86 ± 0.79, 7.36 ±1.17, 8.39 ± 0.99 ve 8.64 ±1.18 nmol/g. YA bulundu. Seruîn linoleik, linolenik ve araşidonik asitler arasındaki korelasyon katsayıları istatistiki olarak önemsiz bulundu. Derialtı yağ dokusu ise II. grupta linoleik- lİnolenik,, linoleik-araşidonik arasında negatif ve V. grupta ise linoleik-araşidonik arasında pozitif korelasyon bulundu. Abdominal yağ dokusunda da, IV. grupta linoleik-araşidonik arasında pozitif korelasyon derecesinde önemliydi (p<0.05). Ön çalışmayla, kazlar için, literatürlerde karşılaşılmayan serum, derialtı ve abdominal dokudaki esansiyel yağ asitlerinin normal değerleri tesbit edildi.

Özet (Çeviri)

60 groups 9.94 ± 1.22, 12.75 ± 1.57, 21.47 ± 3.62 and 7.65 ± 0.86 nmol/g wet wt respectively, and the difference between groups I-IV and IV-V. was significant (p<0.01). Arachidonic acid in the control group 8.32 ± 0.86 nmol/g wet wt in experimental groups 8.86 ± 0.79, 7.36 ± 1.17, 8.39 ± 0.99 ve 8.64 ± 1.18 nmol/g wet wt respectively. The correlation coefficient among serum linolic, linolenic and arachidonic acid was found to be in significant In the subcutan fatty tissue of II. group correlation between linoleic-linolenic, linoleic-arachidonic acids was negative, and of V. group between linoleic-arachidonic acids it was positive. In the abdominal fatty tissue of I st group was found to be significant (p<0.05) between linoleic-arachidonic acids. With a preliminary study nornal values which are not found in literature of essantial fatty acids in the serum, subcutan and abdominal tissue were determinated for the geese.59 significant statistically between groups I-IV, III-V and IV-V.(p<0.0 1). Average values of serum linoleic acid in the control group 35.09 ± 1.79 nmol/ml, in experimental groups 40.77 ± 3.32, 47.32 ± 4.09, 63.50 ± 7.05 and 27.48 ± 2.35 nmol/ml respectively, and the difference between groups I-IV, II-IV, II- V, ni-IV, III- V and IV-V. was significant (p<0.01). Linolenic acid in the control group 22.90 ± 2.27 nmol/ml, in experimental groups 24.20 ± 2.09, 36.52 ± 3.76, 49.18 ± 4.26 and 20.66 ± 2.56 nmol/ml respectively, and the difference between groups I-in, I-IV, II- in, H-IV, in-IV, III-V and IV-V. was significant (p<0.01). Arachidonic acid in the control group 10.57 ± 0.86 nmol/ml, in experimental groups 10.70 ± 0.83, 9.04 ± 0.64, 5.80 ± 0.90 and 9.48 ±1.11 nmol/ml respectively, and the difference between groups I-m, I-IV, H-in, II-IV, in-IV, m-V and IV-V. was found to be significant (pO.01). Average values of linoleic acid in subcutan fatty tissue in the control group 14.74 ± 1.8 nmol/g wet wt, in experimental groups 16.23 ± 1.16, 23.42 ± 2.85, 34.63 ± 3.57 and 8.95 ± 0.82 nmol/g wet wt respectively, and the difference between groups I-in, I-IV, II-III, H-IV, H-V, in-IV and in-V. was found to be significant (p<0.01). Linolenic acid in the control group 8.42 ± 0.81 nmol/g wet wt, in experimental groups 8.90 ± 0.70, 17.21 ± 1.33, 22.92 ± 2.31 and 6.98 ± 0.37 nmol/g wet wt respectively, and the difference between groups I-III, I-IV, II-III, II-IV, III- IV, III-V and IV-V. was significant (p<0.01). Arachidonic acid in the control group 5.59 ± 0.60 nmol/g wet wt in experimental groups 5.13 ± 0.67, 5.45 ± 0.57, 4.20 ± 0.42 ve 6.76 ± 0.84 nmol/g wet wt respectively. Average values of linoleic acid in abdominal fatty tissue in the control group 19.58 ± 2.37 nmol/g wet wt, in experimental groups 28.27 ± 4.75, 49.0 ± 11.27, 61.01 ± 9.98 and 18.40 ± 1.15 nmol/g wet wt respectively, and the difference between groups I-III, I-IV, II-IV and IV-V. was found to be significant (pO.01). Linolenic acid in the control group 8.95 ± 0.76 nmol/g wet wt, in experimental60 groups 9.94 ± 1.22, 12.75 ± 1.57, 21.47 ± 3.62 and 7.65 ± 0.86 nmol/g wet wt respectively, and the difference between groups I-IV and IV-V. was significant (p<0.01). Arachidonic acid in the control group 8.32 ± 0.86 nmol/g wet wt in experimental groups 8.86 ± 0.79, 7.36 ± 1.17, 8.39 ± 0.99 ve 8.64 ± 1.18 nmol/g wet wt respectively. The correlation coefficient among serum linolic, linolenic and arachidonic acid was found to be in significant In the subcutan fatty tissue of II. group correlation between linoleic-linolenic, linoleic-arachidonic acids was negative, and of V. group between linoleic-arachidonic acids it was positive. In the abdominal fatty tissue of I st group was found to be significant (p<0.05) between linoleic-arachidonic acids. With a preliminary study nornal values which are not found in literature of essantial fatty acids in the serum, subcutan and abdominal tissue were determinated for the geese.

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