Matrix entrapment of glucose oxidase by gamma irradiation
Başlık çevirisi mevcut değil.
- Tez No: 6798
- Danışmanlar: PROF. DR. VASIF N. HASIRCI
- Tez Türü: Yüksek Lisans
- Konular: Biyoloji, Biology
- Anahtar Kelimeler: Blucose oxidase, Enzyme immobilization, Bamma irradiation, N~Vinyl pyrrol idone, 2-Hydroxyethyl met hac ry late- IV
- Yıl: 1989
- Dil: İngilizce
- Üniversite: Orta Doğu Teknik Üniversitesi
- Enstitü: Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 102
Özet
glikoz oksidazin gamma işinlamasiyla matrîste tutuklanması Gürsel, ihsan Fen ve Edebiyat Fakültesi Biyoloji Bölümü, Y. Lisans Tezi Tez Yöneticisi s Prof. Dr. Vasıf W. Hasırcı 102 Sayfa, Eylül 1989 ÖZET Endüstride kullanım potansiyeli yüksek olan glikoz oksidaz enziminin tutuklanması proses giderlerinin düşürülmesi yönünden büyük önem taşır. Bu çalışmada glikoz oksidazin matris içinde tutuklanması, N- vinil pirolidon CNVPî, 2-hidraksietil metakrilat
Özet (Çeviri)
MATRIX ENTRAPMENT OF GLUCOSE OXIDASE BY GAMMA IRRADIATION GÜRSEL, İHSAN Faculty of Arts and Sciences Department of Biology, M=Sc. Thesis Supervisor: Prof.Dr. Vasıf N. Hasırcı 102 Pages, September 1989 ABSTRACT Glucose oxidase is an enzyme which has great in dustrial potential and thus its immobilization is of ut most importance in cost reduction- In this study matrix entrapment of glucose oxidase was achieved using gamma irradiation of monomers N-vinyl pyrrol idone (NVP), 2- hydroxyethyl methacrylate CHEMA), and their mixture. In order to test the effect of radiation on entrapment efficiency, retention of activity and physical properties of the system, duration and temperature were varied. Reusability of the resultant products were tested- A com mercially available support suggested for covalent chemi cal linkage was also used for glucose oxidase binding and the results obtained were used for comparison with those from the matrix entrapment- It was generally found that inclusion of the hydrophilic monomer NVP into the matrix increases.the -III-water content, entrapment efficiency and enzyme activity» Irradiation by gamma did not have a detrimental effect on the activity of pure enzyme when exposure was --196*0 but this radioresistance was less at higher temperatures. At 0°C and RT increasing the radiation dose increased the radiation damage» Freeze-thaw cycle did not affect enzyme activity» IR spectroscopy indicated that there was no chemical binding between the matrix and the enzyme» Repeated use studies revealed a gradual loss of activity in all of the samples tested for 10 runs» Finally the commercial sample. VA-Epoxy beads, had high enzyme bind ing but extremely low bound enzyme activities.
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