Aspiration-assisted bioprinting
Başlık çevirisi mevcut değil.
- Tez No: 719866
- Danışmanlar: PROF. DR. İBRAHİM TARIK ÖZBOLAT
- Tez Türü: Doktora
- Konular: Mühendislik Bilimleri, Makine Mühendisliği, Engineering Sciences, Mechanical Engineering
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2022
- Dil: İngilizce
- Üniversite: The Pennsylvanıa State Unıversıty
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
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Özet (Çeviri)
Each year there are approximately 130,000 organ transplants conducted worldwide. Although a substantial number of organ transplantation, the current situation is far away from meeting the demand so that many people on the waitlist die each day. The supply of transplantable tissue faces the same complications and relies on a series of grafting techniques. Allografts (tissue from another human) and xenografts (tissue from an animal) are used to increase supply but these methods have a variety of issues. These problems include the possibility of rejection due to the presence of specific histocompatibility antigens that are targeted by antibodies in the recipient, and for the later there is a risk of microbial or viral transmission resulting in novel diseases. Autografts (tissue from the patient) eliminate the concerns of the previous two methods thanks to the use of genetically identical tissues. However, Autografts are only applicable in a small number of cases due to a lack of supply and large cost associated with the procedure. There is a clear need for cheap, accessible, and biocompatible tissues and organs for transplantation with a possible solution lying in bioprinting. Bioprinting offers a promising technology to fuse the high precision and time efficient qualities of additive manufacturing with tissue engineering to replicate morphogenesis with the ultimate goal of producing artificial tissues and organs on an industrial scale. This dissertation has centered on understanding, designing, and prototyping a new hybrid bioprinting process, called“Aspiration-assisted Bioprinting (AAB),”which enables to pick and precisely bioprint a wide of range of biologics, with dimensions in an order-of-magnitude range (80-800 μm), into/onto a gel substrate with minimal cellular damage. In order to better understand the response of biologics to the bioprinting process, the underlying mechanism explaining the physical behavior of viscoelastic spheroids and their interactions with physical governing forces during aspiration, lifting and bioprinting were unveiled. Compared to existed methods, the approach that was explored in this dissertation facilitated the bioprinting of spheroids in higher iv positional precision and accuracy, ~11 and ~15% with respect to the spheroid size, respectively. In addition, it enabled bioprinting in 3D with flexibility of bioprinting of non-uniform, any-size spheroids into (I) a functional gel as a scaffold-based approach or (II) a sacrificial gel as a scaffoldfree approach. The newly-developed AAB platform, modified from a Makerbot Replicator I (<$1,000), operates a custom-made glass pipette, which is used to 'pick up' biologics and '3D bioprint' them into or onto a gel substrate. This new approach paves the way for bioprinting several tissue types and a wide range of spheroids in 2D and 3D in gel substrates with novel applications including (I) the development of micro-physiological systems to demonstrate the collective angiogenic sprouting behavior of spheroids in a scaffold medium, (II) fabrication of osteogenic tissues to decode the role of mid-term osteogenic induction of stem cell-based spheroids (prior to bioprinting) on the mineralization and assembly behavior in a scaffold-free environment and (III) fabrication of the 3D osteochondral interface with biologically-relevant morphologies, which is quite challenging using existing technologies
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