In vitro oksidatif hasar oluşturulmuş DNA üzerine nar kabuğu, tetra ve zeytin yaprağı ekstraktlarının etkisinin incelenmesi
The investigation of effect of pomegranate peel, tetra and olive leaf extracts on DNA oxidatively damaged in vitro
- Tez No: 754412
- Danışmanlar: PROF. DR. ŞEBNEM SELEN İŞBİLİR
- Tez Türü: Doktora
- Konular: Kimya, Chemistry
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2022
- Dil: İngilizce
- Üniversite: Trakya Üniversitesi
- Enstitü: Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Kimya Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 149
Özet
Çalışmanın amacı besin olarak kullanılmayan nar (Hicaz türü) kabuğu, zeytin yaprağı (Domal ve Gemlik türleri) ve tetra yaprağında (Cotinus coggygria) elde edilen ekstraktların antioksidan aktivitelerine katkı sağlayan fıtobileşenlerce analizlenmesi, bu bitki örneklerinin DNA üzerind oluştunılan in vitro oksidatif hasar üzerinde koruyucu etkisinin, karaciğe sağlıklı ve kanser hücre hattı üzerinde sitotoksik etkisinin olup olmadığını incelenmesidir.
Özet (Çeviri)
This study aims to investigate the extracts obtained from pomegranate peel (Hicaz gentype), olive leaves (Domat ve Gemlik gentypes) and smoke tree leaf (Cotinus coggygria), which are not used as nutrients, by phytocomponents that contribute to their antioxidant activities, and to determine whether these plant samples have protective effect agains1 oxidative DNA damage. Different extraction techniques are used to obtain phenolic-ricb extract from plant sources. Both conventional extraction technique using a shaking water batlı and ultrasound-assisted extraction as a modem method f\,vere performed with 70% ethanol solvent for extractions of pomegranate peel, smoke tree and olive leaves. It was observed that the extracts obtainec from ultrasonic assisted extraction (USE) generally had higher extract yield, free radical scavenging capacity and, total phenolic and flavonoic compounds carried out by spectrophotometric method. In DPPH and ABTS methods, smoke tree leaf and pomegranate peel had low EC50 values which indicates the strong free radicals scavenging activities. MTT assay was applied to the extracts using AML12 (normal liver) and Hep3B (liver cancer) celi lines. Cells were treated with extracts o different concentrations during 24 hours and 48 hours. The extracts o smoke tree leaf, Hicaz type pomegranate peel, Gemlik olive leaf and Mix (pomegranate peel + smoke tree leaf + Domat type olive leaf mixture) showed a dose-dependent protective effect on AML 12 cells. Domat olivı:: leaf extract, on the other hand, caused a decrease in viability at the higl: concentrations studied. A dose-dependent cytotoxic effect was observed in Hep3B cells, after 24 hours of incubation, ali extracts, especially smoke tree leaf and Mix exh·act, showed antiproliferative effects, except for Doma1 sample. Antioxidants are compounds or herbal sources that prevent the formation and harmful effects of free radicals and, asa result, protect DNA and other biomolecules against oxidative stress. Measurement of 8-hydroxy 2'-deoxyguanosine (8-OH-2dG) and/or 8-hydroxyguanine (8-OHGua) an markers of oxidative damage in DNA. In the study, the hydroxyl radica formed by Fenton reaction damaged calf thymus DNA oxidatively and, thı:: amounts of 8-OH-2dG were measured by LC-MS/MS. The capacity o ıoomegranate peel, smoke tree leaf, Domat and Gemlik olive leaf extracts and the Mix extract to prevent this damage was evaluated. Hica:1 pomegranate peel, smoke tree leaf and Mix extracts prevented oxidative DNA damage at the highest rate. The use of plant products that are not consumed as food oı agricultural waste products as a source of bioactive compounds is importan in terms of economic and recovery. The phenolic compounds and quantity determinations of the plants used in the study were made by LC- MS/MS. The Hicaz pomegranate peel contains ellagic acid as majoı compound, abscisic acid, ethy 1 gallate, gallic acid. Smoke tree leaf contains he most verbascoside, gallic acid, protocatechic acid, myricetin, ethyl gallate and syringic acid. In olive leaves, mainly oleuropein, chlorogenic acid, verbascoside, tyrosinol and rutin were found. These bioactive compounds, which are secondary metabolites of plants, may be responsible for their antioxidant, cytotoxic and oxidative DNA damage prevention activities.
Benzer Tezler
- Farelerde dobutamin ile oluşturulmuş kalp hasarı üzerine fuzilin' in koruyucu etkileri
Protective effects of fuziline on dobutamine-induced heart injury in mi̇ce
YASEMİN HACANLI
Doktora
Türkçe
2022
Göğüs Kalp ve Damar CerrahisiHarran ÜniversitesiKalp ve Damar Cerrahisi Ana Bilim Dalı
PROF. DR. MEHMET SALİH AYDIN
- Dakapo üzümü posası ekstraktının V79 hücreleri üzerine antigenotoksik etkilerinin incelenmesi
Investigation of antigenotoxic effects of dakapo grape pomace extract on V79 cells
EZGİ EYLÜL BANKOĞLU
Yüksek Lisans
Türkçe
2011
Gıda MühendisliğiEge ÜniversitesiGıda Mühendisliği Ana Bilim Dalı
PROF. DR. TOMRİS ONOĞUR
- Elektromanyetik radyasyonun (2.45 GHz) rat beyninde oluşturduğu oksidatif hasar ve sarımsağın koruyucu etkisi
Rat brain oxidative stress induced by the electromagnetic radiation (2.45 GHz) and the protective effect of garlic
HATİCE ŞİMAL GÜRLER
Tıpta Uzmanlık
Türkçe
2012
BiyokimyaOndokuz Mayıs ÜniversitesiTıbbi Biyokimya Ana Bilim Dalı
YRD. DOÇ. BİRŞEN BİLGİCİ
- Psöriasiste darbant ultraviyole-B ve E vitamininin mutajenik risk üzerine etkilerinin comet tekniği ile değerlendirilmesi
'study with comet assay, the effect of narrowbant uv-B and vitamin E on mutagenic risk in psoriasis vulgaris patients??
CANAN KEVLEKÇİ
Tıpta Uzmanlık
Türkçe
2008
DermatolojiGazi ÜniversitesiDermatoloji Ana Bilim Dalı
PROF. DR. AYLA GÜLEKON
- Karaciğer karsinomu (HepG2) hücrelerinde, cisplatin toksikasyonuna arbutin etkileri
The effects of arbutin on cisplatin toxicity in liver carcinoma (HepG2) cell line
HATİCE EVİN