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In vitro oksidatif hasar oluşturulmuş DNA üzerine nar kabuğu, tetra ve zeytin yaprağı ekstraktlarının etkisinin incelenmesi

The investigation of effect of pomegranate peel, tetra and olive leaf extracts on DNA oxidatively damaged in vitro

  1. Tez No: 754412
  2. Yazar: DUA DANJOLLİ-HASHANİ
  3. Danışmanlar: PROF. DR. ŞEBNEM SELEN İŞBİLİR
  4. Tez Türü: Doktora
  5. Konular: Kimya, Chemistry
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2022
  8. Dil: İngilizce
  9. Üniversite: Trakya Üniversitesi
  10. Enstitü: Fen Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Kimya Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 149

Özet

Çalışmanın amacı besin olarak kullanılmayan nar (Hicaz türü) kabuğu, zeytin yaprağı (Domal ve Gemlik türleri) ve tetra yaprağında (Cotinus coggygria) elde edilen ekstraktların antioksidan aktivitelerine katkı sağlayan fıtobileşenlerce analizlenmesi, bu bitki örneklerinin DNA üzerind oluştunılan in vitro oksidatif hasar üzerinde koruyucu etkisinin, karaciğe sağlıklı ve kanser hücre hattı üzerinde sitotoksik etkisinin olup olmadığını incelenmesidir.

Özet (Çeviri)

This study aims to investigate the extracts obtained from pomegranate peel (Hicaz gentype), olive leaves (Domat ve Gemlik gentypes) and smoke tree leaf (Cotinus coggygria), which are not used as nutrients, by phytocomponents that contribute to their antioxidant activities, and to determine whether these plant samples have protective effect agains1 oxidative DNA damage. Different extraction techniques are used to obtain phenolic-ricb extract from plant sources. Both conventional extraction technique using a shaking water batlı and ultrasound-assisted extraction as a modem method f\,vere performed with 70% ethanol solvent for extractions of pomegranate peel, smoke tree and olive leaves. It was observed that the extracts obtainec from ultrasonic assisted extraction (USE) generally had higher extract yield, free radical scavenging capacity and, total phenolic and flavonoic compounds carried out by spectrophotometric method. In DPPH and ABTS methods, smoke tree leaf and pomegranate peel had low EC50 values which indicates the strong free radicals scavenging activities. MTT assay was applied to the extracts using AML12 (normal liver) and Hep3B (liver cancer) celi lines. Cells were treated with extracts o different concentrations during 24 hours and 48 hours. The extracts o smoke tree leaf, Hicaz type pomegranate peel, Gemlik olive leaf and Mix (pomegranate peel + smoke tree leaf + Domat type olive leaf mixture) showed a dose-dependent protective effect on AML 12 cells. Domat olivı:: leaf extract, on the other hand, caused a decrease in viability at the higl: concentrations studied. A dose-dependent cytotoxic effect was observed in Hep3B cells, after 24 hours of incubation, ali extracts, especially smoke tree leaf and Mix exh·act, showed antiproliferative effects, except for Doma1 sample. Antioxidants are compounds or herbal sources that prevent the formation and harmful effects of free radicals and, asa result, protect DNA and other biomolecules against oxidative stress. Measurement of 8-hydroxy 2'-deoxyguanosine (8-OH-2dG) and/or 8-hydroxyguanine (8-OHGua) an markers of oxidative damage in DNA. In the study, the hydroxyl radica formed by Fenton reaction damaged calf thymus DNA oxidatively and, thı:: amounts of 8-OH-2dG were measured by LC-MS/MS. The capacity o ıoomegranate peel, smoke tree leaf, Domat and Gemlik olive leaf extracts and the Mix extract to prevent this damage was evaluated. Hica:1 pomegranate peel, smoke tree leaf and Mix extracts prevented oxidative DNA damage at the highest rate. The use of plant products that are not consumed as food oı agricultural waste products as a source of bioactive compounds is importan in terms of economic and recovery. The phenolic compounds and quantity determinations of the plants used in the study were made by LC- MS/MS. The Hicaz pomegranate peel contains ellagic acid as majoı compound, abscisic acid, ethy 1 gallate, gallic acid. Smoke tree leaf contains he most verbascoside, gallic acid, protocatechic acid, myricetin, ethyl gallate and syringic acid. In olive leaves, mainly oleuropein, chlorogenic acid, verbascoside, tyrosinol and rutin were found. These bioactive compounds, which are secondary metabolites of plants, may be responsible for their antioxidant, cytotoxic and oxidative DNA damage prevention activities.

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