In-vitro effects of orange peel extracts on candida albicans, a major source of fungal infections
Başlık çevirisi mevcut değil.
- Tez No: 797084
- Danışmanlar: DR. ÖĞR. ÜYESİ ŞEBNEM GARİP USTAOĞLU
- Tez Türü: Yüksek Lisans
- Konular: Biyofizik, Biyokimya, Mikrobiyoloji, Biophysics, Biochemistry, Microbiology
- Anahtar Kelimeler: Candida Albicans, Orange Peel Extracts, Fungal Infections, FTIR Spectroscopy
- Yıl: 2022
- Dil: İngilizce
- Üniversite: Altınbaş Üniversitesi
- Enstitü: Lisansüstü Eğitim Enstitüsü
- Ana Bilim Dalı: Biyomedikal Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 94
Özet
Although it is a normal component of our microbiota, Candida albicans can also cause systemic and mucosal infections, which in those with diabetes, malnutrition, and immune system compromises can be fatal. These are the most prevalent species of nosocomial pathogens that can become resistant to antifungal medicines. The findings of this study also shown the capability of ATR-FTIR spectroscopy to discern between the antifungal effects of various doses of orange extract samples on the species composition of Candida albicans.
Özet (Çeviri)
Candida albicans is the part of our natural microflora but also responsible for mucosal and systematic infections that can lead to death in individuals with malnutrition, diabetes and immunocompromised individuals. These are the most common species which cause nosocomial diseases and are capable of developing resistance to antifungal antibiotics. The mechanism of action of antifungal drugs to be used against fungal infections are different from antibacterial drugs as fungi are eukaryotes and bacteria are prokaryotes. It is the first study to identify the dose range with the antifungal effect of various orange peel extracts in Candida fungus and the most appropriate orange extract to be studied using infrared spectroscopy with chemometric analysis, to find out the structural and functional effects of orange extract in fungal biomolecules with detailed spectral characterization. Thus, a novel method that can monitor the antifungal effect can be proposed with this study. Based on the results of disc diffusion and microtubule dilution tests, we determined the concentration of the orange extract that we use for IR spectrum analysis which are OE50%, OE25%, OE12.5%, OE6% and two other groups named control (C) along with Candida (CA). Different orange extract doses can show antifungal effects on Candida albicans by effecting the molecular structure and composition of the components of yeast. The most effected components were seen as saturated and unsaturated lipids with triglycerides. In contrast, the protein components of yeast seem to be not affected significantly from the antifungal effects of OE. There is a dose difference in the antifungal effects of orange extract on Candida albicans samples. The best doses for the antifungal activity were determined as medium doses (25 % and 12,5 %) especially 25% OE dose. The highest (50%) and lowest (6%) doses show opposite effects on yeast when compared to the medium doses. The optimal antifungal effect was seen with medium OE doses (12,5% and 25%) by affecting the saturated and unsaturated lipid concentration and composition of C. albicans. The protein changes were related to the changes of lipid metabolism of yeast related to the enzymes and regulatory proteins of that metabolism. There was no dose effect in the changes of nucleic acid and carbohydrate contents except low dose of OE (6%) which shows opposite effect for those parameters when compared to the other doses of OE. The results of this study also showed that ATR-FTIR spectroscopy has the capacity to distinguish the antifungal effects of different doses of orange extract samples on the composition of Candida albicans species.
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