Effect of rigid gas permeable (RPG) contact lens care solutions oncultured epithelial cells
Başlık çevirisi mevcut değil.
- Tez No: 917285
- Danışmanlar: DR. MAY M. BAKKAR
- Tez Türü: Yüksek Lisans
- Konular: Belirtilmemiş.
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2017
- Dil: İngilizce
- Üniversite: University of Jordan (Al-Jami'ah Al-Urdunia)
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 65
Özet
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Özet (Çeviri)
Background: Contact lenses are appliances that clinically used to correct ocular refractive errors as an alternative management option to spectacles correction. There are two main types of contact lenses that are used extensively today. These include soft contact lenses (SCL) and rigid gas permeable (RGP) contact lenses. Contact lens care solutions are chemical formulas that used by contact lens wearers along with contact lens use in order to clean the lens from debris and dirt, wet the lens surface and disinfect the lens from microbes. Multipurpose solutions (MPSs) which are named due to the different purposes of their use (cleaning, wetting, and disinfection) are considered the main type of contact lens care solutions. During contact lens wear, contact lenses that have been soaked overnight in MPS are applied to the ocular surface (the most anterior part of the eye). It is suggested that a trace amount of MPS could enter into the corneal cells upon contact lens insertion and through continuous release from the lens. It has been reported that everyday usage of MPS for long term to clean and disinfect contact lenses may result in toxicity and adverse effect on the corneal epithelial cells. Such possible toxicity has been extensively reported during soft contact lens wear. However, the safety of RGP contact lens solutions is poorly studied. Purpose: In this study, epithelial cell culture was used to investigate and compare possible toxicity of MPSs that used with RGP lenses. Methods: Cultured epithelial cells were treated with different types of RGP MPSs and an appropriate control was used. Cytotoxicity assay such as 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was used to study possible toxic effect caused by MPSs against the cells. Additionally, cell cycle analysis using flowcytometry was used to assess the impact of MPSs on cell cycle profile. Wound healing model was also used to study the effect of different MPSs on cell migration during healing process. Results: The results show a higher toxic effect of polyhexanide 0.0005% and chlorhexidine-based MPS compared to others tested solution. Some toxic effects seem to be reversible as it is observed after re-growing the MPSs-treated cells in fresh cell culture media.
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