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Mineral trioxide aggregate'in sitotoksik ve antimikrobiyal özelliklerinin değerlendirilmesi

Evaluation of cytotoxic and antimicrobial properties of mineral trioxide aggregate

  1. Tez No: 163996
  2. Yazar: EMİNE ŞEN TUNÇ
  3. Danışmanlar: PROF.DR. SERAP ÇETİNER
  4. Tez Türü: Doktora
  5. Konular: Diş Hekimliği, Dentistry
  6. Anahtar Kelimeler: Antimicrobial activity, biocompatibility, cell culture, cytotoxicity, mineral trioxide aggregate, Antimicrobial activity, biocompatibility, cell culture, cytotoxicity, mineral trioxide aggregate
  7. Yıl: 2005
  8. Dil: Türkçe
  9. Üniversite: Ankara Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Pedodonti Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 166

Özet

132 SUMMARY Evaluation of Cytotoxic and Antimicrobial Properties of Mineral Trioxide Aggregate The aim of this in vitro study was to evaluate the cytotoxic and antimicrobial properties of recently introduced material, mineral trioxide aggregate (MTA). In the first part of the study, cytotoxic properties of MTA were assessed by using agar overlay, millipore filter and extraction test methods. While cytotoxic effects of white MTA and calcium hydroxide were compared by using agar overlay and millipore filter test methods; extraction test methods were used to compare the cytotoxic effects of two forms of MTA [0]and calcium hydroxide.[0] Human permanent tooth pulp fibroblasts were used as a biological system in the agar overlay and millipore filter test methods. However, in the extraction test[0]s, the assessments were made by using both human permanent tooth pulp fibroblasts and VERO cells.[0] In all test methods, a positive correlation was found between time and cytotoxicity. When the toxic zone diameter findings of fresh and 24 hours set specimens of white MTA and calcium hydroxide which were tested with agar overlay and millipor filter methods were compared, it was observed that the fresh specimens were more cytotoxic than the set specimens in all incubation periods.to] Comparison of the white and gray MTA with the extraction tests, both microscopic and cell viability results had shown that white MTA was more cytotoxic than gray MTAm. In the extraction test[o]S, the dilution rates, treatment time and material variables had affected the survival rates of both human permanent pulp fibroblasts and VERO cells similarly. When the percentages of survival rates were compared, the results were more sensitive in VERO which is a permanent cell line. However, it was thought that cell numerical differences of survival rates were not enough important to effect the cytotoxicity degrees of the materials. In all tests which were aimed to evaluate the cytotoxic properties of test materials, calcium hydroxide was found to be less cytotoxic than the two forms of MTA. However, when the cytotoxic features of the test materials were ranked, all materials showed slight cytotoxic features during the determined incubation period.133 In the second part of the study, antimicrobial properties of MTA were evaluated by using agar diffusion test method. Antimicrobial properties of two forms of MTA and calcium hydroxide were investigated on four facultative bacteria. Calcium hydroxide did not show any antimicrobial effect for all tested bacteria in all incubation periods. Although both forms of MTA were effective on the same bacteria, the results showed that white MTA was more effective than the other. According to the results of this in vitro study, it was thought that clinic application results of two different types of MTA could have the same clinical outcome and these two materials could be used as an alternative for calcium hydroxide. However, controlled clinical trials are necessary to determine any clinical implications of MTA.

Özet (Çeviri)

132 SUMMARY Evaluation of Cytotoxic and Antimicrobial Properties of Mineral Trioxide Aggregate The aim of this in vitro study was to evaluate the cytotoxic and antimicrobial properties of recently introduced material, mineral trioxide aggregate (MTA). In the first part of the study, cytotoxic properties of MTA were assessed by using agar overlay, millipore filter and extraction test methods. While cytotoxic effects of white MTA and calcium hydroxide were compared by using agar overlay and millipore filter test methods; extraction test methods were used to compare the cytotoxic effects of two forms of MTA [0]and calcium hydroxide.[0] Human permanent tooth pulp fibroblasts were used as a biological system in the agar overlay and millipore filter test methods. However, in the extraction test[0]s, the assessments were made by using both human permanent tooth pulp fibroblasts and VERO cells.[0] In all test methods, a positive correlation was found between time and cytotoxicity. When the toxic zone diameter findings of fresh and 24 hours set specimens of white MTA and calcium hydroxide which were tested with agar overlay and millipor filter methods were compared, it was observed that the fresh specimens were more cytotoxic than the set specimens in all incubation periods.to] Comparison of the white and gray MTA with the extraction tests, both microscopic and cell viability results had shown that white MTA was more cytotoxic than gray MTAm. In the extraction test[o]S, the dilution rates, treatment time and material variables had affected the survival rates of both human permanent pulp fibroblasts and VERO cells similarly. When the percentages of survival rates were compared, the results were more sensitive in VERO which is a permanent cell line. However, it was thought that cell numerical differences of survival rates were not enough important to effect the cytotoxicity degrees of the materials. In all tests which were aimed to evaluate the cytotoxic properties of test materials, calcium hydroxide was found to be less cytotoxic than the two forms of MTA. However, when the cytotoxic features of the test materials were ranked, all materials showed slight cytotoxic features during the determined incubation period.133 In the second part of the study, antimicrobial properties of MTA were evaluated by using agar diffusion test method. Antimicrobial properties of two forms of MTA and calcium hydroxide were investigated on four facultative bacteria. Calcium hydroxide did not show any antimicrobial effect for all tested bacteria in all incubation periods. Although both forms of MTA were effective on the same bacteria, the results showed that white MTA was more effective than the other. According to the results of this in vitro study, it was thought that clinic application results of two different types of MTA could have the same clinical outcome and these two materials could be used as an alternative for calcium hydroxide. However, controlled clinical trials are necessary to determine any clinical implications of MTA.

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