Deneysel olarak yapılan karaciğer rezeksiyonu sırasında iskemik ön koşullama yapılan ratlara hepatektomi öncesi verilen slimarin'in karaciğer rejenerasyonu üzerine etkisi
Silymarin and ischemic preconditioning effects of liver regeneration on experimental liver resection in rats
- Tez No: 431701
- Danışmanlar: PROF. DR. AYDIN DALGIÇ
- Tez Türü: Tıpta Uzmanlık
- Konular: Genel Cerrahi, General Surgery
- Anahtar Kelimeler: Karaciğer Rezeksiyonu, Silimarin, Liver resection, Silymarin, Ischemic preconditioning
- Yıl: 2016
- Dil: Türkçe
- Üniversite: Gazi Üniversitesi
- Enstitü: Tıp Fakültesi
- Ana Bilim Dalı: Genel Cerrahi Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 98
Özet
Özet yok.
Özet (Çeviri)
Introduction: Many surgical techniques and medical or herbal drugs have been experimented in order to increase regeneration capacity of the liver and experiments are still in course. Therefore, we have aimed to examine the effects of silymarin, which is administrated to the rats implemented with IPC before hepatectomy during the experimental liver resection, on the liver regeneration. Materials and Methods: The study has been started after receiving consent, from Local Ethics Committee for Animal Experiments of Gazi University, dated 26/06/2015 and coded G.Ü.ET-15.047. In the study, 30 male Wistar-Albino rats have been used. On the rats in the sham group, only hepatic manipulation and portal triad dissection have been implemented; and they have been sacrificed after 48 hours. On Group II (Partial hepatectomy), 70% liver resection has been implemented. Resected liver tissue has been weighed. Rats have been sacrificed after 48 hours. On Group III (Silymarin + Partial hepatectomy), 200 mg/kg of Silymarin dose have been implemented by adding it to their drinking water for six weeks. Six weeks later, 70% liver resection has been implemented on rats; and rats have been sacrificed after 48 hours. On Group IV (Ischemic preconditioning + Silymarin + Partial hepatectomy), Silymarin is administered for 6 weeks before the process, and then 70% liver resection has been implemented after ischemic preconditioning; and rats have been sacrificed after 48 hours. On Group V (Ischemic preconditioning + Partial Hepatectomy), after the ischemic preconditioning, 70% liver resection has been implemented; and rats have been sacrificed after 48 hours. After the hepatectomies implemented on rats, serum AST, LDH, Bilirubin, TNF-α and IL-6 values have been studied with ELISA method. Tissue samples have been histopathologically evaluated in terms of a number of mitosis, congestion, necrosis, cytoplasmic vacuolization and presence of neutrophils. PCNA (proliferating cell nuclear antigen) antibodies have been immunohistochemically studied. Results: In the group administered with Silymarin, it has been found out that regeneration capacity of remaining liver tissue had been higher, and liver functions had also been affected lesser compared to the group on which silymarin has not been administered. In addition to this, during the partial hepatectomy, it has been observed that IPC had increased regeneration rate of remaining liver tissue and liver functions had been affected lesser, and, at the same time, it has been found out that silymarin administration before hepatectomy had increased the regeneration rate much more and affected liver functions lesser. Conclusion: With these results, it can be said that silymarin administration and IPC implementation before hepatectomy would increase liver regeneration rate. However, in order for these results should be implemented in the clinic, similar clinical studies are required.
Benzer Tezler
- Deneysel karaciğer iskemi reperfüzyon modelinde Rheum ribes L.(Işgın) koruyucu etkisinin GPER düzeyleriyle değerlendirilmesi
Evaluation of the protective effect of rheum ribes L. in the experimental liver ischemia reperfusion model with the GPER levels
IŞIL YAĞMUR
Tıpta Uzmanlık
Türkçe
2021
BiyokimyaKahramanmaraş Sütçü İmam ÜniversitesiTıbbi Biyokimya Ana Bilim Dalı
PROF. DR. ERGÜL BELGE KURUTAŞ
- İzoniyazidin sıçanlarda oksidatif karaciğer hasarına etkisi ve oksidatif stresin C vitamini ile azaltılması
Başlık çevirisi yok
YAKUP ERGÜL
Tıpta Uzmanlık
Türkçe
2005
Çocuk Sağlığı ve Hastalıklarıİstanbul ÜniversitesiÇocuk Sağlığı ve Hastalıkları Ana Bilim Dalı
PROF.DR. TÜLAY ERKEN
- Karaciğer rezeksiyonu uygulanan ve iskemi reperfüzyon oluşturulan ratlarda glutaminin etkisi
Başlık çevirisi yok
ZAHİDE KURT
Tıpta Uzmanlık
Türkçe
2005
Genel CerrahiEge ÜniversitesiGenel Cerrahi Ana Bilim Dalı
PROF.DR. AHMET ÇOKER
- Parsiyel hepatektomi yapılan sıçanlarda sarımsağın oksidatif hasar ve karaciğer rejenerasyonu üzerine olan etkileri
Impact of allyl disulfide on oxidative damage and liver regeneration in an experimental hepatectomy model
ABDULCABBAR KARTAL
Tıpta Uzmanlık
Türkçe
2013
Beslenme ve DiyetetikSağlık BakanlığıGenel Cerrahi Ana Bilim Dalı
PROF. DR. ADEM AKÇAKAYA
- Deneysel meme kanseri modelinde karaciğer rezeksiyonunun immünolojik etkileri
Immunological effects of liver resection in experimental breast cancer model
HİLMİ ANIL DİNÇER
Tıpta Uzmanlık
Türkçe
2017
Genel CerrahiHacettepe ÜniversitesiGenel Cerrahi Ana Bilim Dalı
PROF. DERYA KARAKOÇ